Journal of Applied Botany and Food Quality <p>This journal is published in collaboration with the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It focuses on applied research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry.</p> en-US Journal of Applied Botany and Food Quality 1613-9216 <p>From Volume 86 (2013) on, the content of the journal is licensed under the&nbsp;<a href="">Creative Commons Attribution-ShareAlike 4.0 License</a>. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and the new work is licensed under a CC-BY-SA compatible license.</p> <p>The copyright of the published work remains with the authors. If you want to use published content beyond what the CC-BY-SA license permits, please contact the corresponding author, whose contact information can be found on the last page of the respective article. In case you want to reproduce content from older issues (before CC BY-SA applied), please contact the&nbsp;corresponding author to ask for permission.</p> Comparison of terpene and phenolic profiles of three wild species of Echeveria (Crassulaceae) <p><span lang="EN-US"><em>Echeveria</em> species (Crassulaceae) are used in traditional medicine and some of their biological activities are demonstrated (e.g. antimicrobial, anti-inflammatory, anticancer). However, their chemical composition has been scarcely studied. The methanol extracts (ME) of three Echeveria species (<em>E. craigiana, E. kimnachii</em> and <em>E. subrigida</em>) from Mexico were analyzed for the sterol (GC-MS) and phenolic (HPLC-DAD-ESI-MSn) composition. Eleven sterols were identified, <em>E. kimnachii</em> showed the highest total content (7.87 mg/g ME), and the main constituents were γ-sitosterol in<em> E. craigian</em>a (33.9%) and <em>E. subrigida</em> (54.4%), and lupenone in <em>E. kimnachii</em> (28.9%). The phenolic analysis showed differences among the Echeveria species, which contained flavonoids derivatives and tannins as the main components. The main flavonoids in <em>E. craigiana</em> were hexoside derivatives of quercetin and isorhamnetin, both with a 3-hydroxy-3-methylglutaroyl substituent; in E. subrigida hexosides of isorhamnetin, quercetin and kaempferol; and<em> E. kimnachii</em> showed the greatest diversity including proanthocyanidins and less common flavonoid derivatives of kaempferol O,O-disubstituted by acyl derivatives. The characteristic phytochemicals of each studied <em>Echeveria</em> species could be responsible of its specific biological activities and useful as chemotaxonomic markers. The kaempferol derivatives in <em>E. kimnachii</em> are rare in nature and they will be isolated and characterized.</span></p> Gabriela López-Angulo Julio Montes-Avila Sylvia Páz Díaz-Camacho Rito Vega-Aviña José Ángel López-Valenzuela Francisco Delgado-Vargas ##submission.copyrightStatement## 2018-06-11 2018-06-11 91 145 154 10.5073/JABFQ.2018.091.020 Genetic resources and selected conservation methods of tomato <p>Tomato is one of the most popular vegetable crops. However, over time, the species has suffered a strong genetic diversity reduction and domestication bottlenecking. This growing trend is known as the genetic erosion. The human intervention on the genetic erosion intensification is high and has severe implications on the future programmes of management and use of <em>S. lycopersicum</em> biodiversity. The wild tomato species (especially accessions originating from the Andes to Mesoamerica) harbour many valuable genes, which have been lost among the cultivated ones. Therefore, there is an increasing interest to mine new alleles from the interspecific gene pool of <em>Lycopersicon</em> section. Sustainable genetic diversity management constitutes a basis for crop improvement, classification and protection. Moreover, conservation of plant genetic resources is crucial to food security, as well as pharmaceutical industry. There are a few strategies developed which address the preservation of tomato genetic resources. In situ and ex situ conservation are the two main complementary methods of biodiversity protection. The aim of this review is to summarise the most recent information about tomato genetic resources, genetic erosion phenomenon, as well as some traditional and modern preservation strategies.</p> Dariusz Kulus ##submission.copyrightStatement## 2018-06-11 2018-06-11 91 135 144 10.5073/JABFQ.2018.091.019 Antineurodegenerative, antioxidant and antibacterial activities and phenolic components of Origanum majorana L. (Lamiaceae) extracts of different origin <p><span lang="EN-GB">The aim of this study was to examine chemical composition, as well as antineurodegenerative, antioxidant and antibacterial activity of aqueous and ethanolic extracts of <em>Origanum majorana</em> L. (Lamiaceae) originating from Serbia, Greece, Egypt and Libya. Total phenolics and flavonoids, antioxidant activities, and acetylcholinesterase and tyrosinase inhibitory activities were measured spectrophotometrically. Determination of phenolic compounds in extracts was done using HPLC-DAD technique. Antibacterial activity included determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using the microdilution method. The highest phenolic and flavonoid contents were recorded in the ethanolic extract of the Egyptian sample and in aqueous extract of Serbian sample. The HPLC analysis showed high content of rosmarinic acid, with the highest amount found in the ethanolic extract of the plants from Egypt. Water extracts showed prevalently better antioxidant and antineurodegenerative activity in applied tests than the ethanolic extracts. Gram-positive bacterial strains showed higher sensitivity to tested extracts. According to the obtained results, sweet marjoram samples from Serbia and Egypt can be marked as more promising, due to the highest content of total phenolics and flavonoids and the best antioxidant, antibacterial and tyrosinase inhibitory activity.</span></p> Sonja Duletić-Laušević Ana Zoran Alimpic Stoimir Kolarević Branka Vuković-Gačić Mariana Oalđe Jelena Živković Katarina Šavikin Petar D. Marin ##submission.copyrightStatement## 2018-06-11 2018-06-11 91 126 134 10.5073/JABFQ.2018.091.018 Analysis of nutritional composition and antioxidant activity of sweet potato (Ipomoea batatas L.) leaf and petiole <p>Two cultivars of sweet potatoes, Pumpkin and Chestnut, were planted in the didactic field of the Faculty of Horticulture, Craiova, and biochemical determinations were carried out from the blades and petioles of the leaves. This study highlights the antioxidant properties of these species, which are not well known in Romania, to encourage consumption of the vegetative parts of the plant, as well as use of the leaves in the future to develop natural antioxidants. The chlorophyll and carotene content in the blade and petiole were determined, highlighting the large amounts of petiole, depending on the cultivar. Catalase (CAT), peroxidase (POX), and vitamin C content, as well as total dry matter (TDM), reducing sugars, phenolic compounds, and antioxidant activity, were also measured. The petioles exhibited high antioxidant activity, with 62.186 μmol TE/g FW in Pumpkin and 95.168 μmol TE/g FW in Chestnut. The cultivars also exhibited differences at the blade level, with high values recorded for the Chestnut cultivar. Positive correlations between total polyphenols and reducing sugars (r = 0.89), antioxidant activity and reducing sugars (r = 0.48), and antioxidant activity and phenolic compounds (r = 0.53) were found. This study demonstrates that sweet potato leaves are a potential, inexpensive and beneficial source of natural antioxidants.</p> Maria Dinu Rodica Soare Cristina Babeanu Gheorghita Hoza ##submission.copyrightStatement## 2018-05-29 2018-05-29 91 120 125 10.5073/JABFQ.2018.091.017 A novel estimation method of total flavonoids in an edible medicinal mulberry leaves by ultrasound-assisted hydroalcohol-acid extraction and HPLC-DAD <p>Mulberry leaves have been widely used to produce various health products. In this paper, an efficient procedure of ultrasound-assisted hydroalcohol-acid extraction (UAHAE) was established to estimate the total flavonoids content in mulberry leaves by quantifying their resulting aglycones (quercetin and kaempferol) using HPLC-DAD. Effective hydrolysis of glycosides to aglycones was achieved in an ethanol-HCl-water (7/2/1, v/v/v) solution at 75°C by ultrasound (40 kHz) for 60 min. The average contents of quercetin and kaempferol were 7.12 mg/g and 2.13 mg/g, respectively, in the variety of cultivar MC308, and their recoveries were 105.48% and 105.81%, respectively. The total flavonoid content of most varieties was between 4-10 mg/g, accounting for 80% in 86 species of mulberry leaves. With the increase in leaf maturity, the general trend of the change in flavonoid content was a decrease at first and then an increase. The highest total flavonoid content in commercial mulberry tea (CT 2) is 4.76 mg/g, and the DPPH radical scavenging activities were similar to the distribution of total flavonoids measured by UAHAE in three types of mulberry tea. These finding provide a basis for industrial-scale manufacturing of edible medicinal mulberry leaf products.</p> Jin-Ge Zhao Yu-Qing Zhang ##submission.copyrightStatement## 2018-05-16 2018-05-16 91 114 119 10.5073/JABFQ.2018.091.016