Rapid detection of phosphine resistance in the lesser grain borer, <i>Rhyzopertha dominica</i> (Coleoptera: Bostrychidae) from China using ARMS-PCR

Poster

Autor/innen

  • Yujie Lu College of Food, Science and Technology, Henan University of Technology, Zhengzhou, Henan Province, China. 450001
  • Chenguang Zhang College of Food, Science and Technology, Henan University of Technology, Zhengzhou, Henan Province, China. 450001
  • Zhenyan Wang College of Food, Science and Technology, Henan University of Technology, Zhengzhou, Henan Province, China. 450001
  • Xiaoping Yan Sinograin Chengdu Grain Storage Research Institute Chengdu, Sichuan Province, China. 610091
  • Robert N. Emery Biosecurity and Regulation, Entomology Branch Agriculture and Food Department of Primary Industries and Regional Development Australia Perth, WA 6151

DOI:

https://doi.org/10.5073/jka.2018.463.229

Schlagworte:

phosphine resistance, lesser grain borer, taqman@ probe, ARMS-PCR, dihydrolipoamide dehydrogenase

Abstract

MThe lesser grain borer, Rhyzopertha dominica is one of the serious cosmopolitan stored grain pests worldwide. High phosphine resistant R. dominica has been reported in several countries. The evolution of strong phosphine resistance is a major challenge for continuous application of the fumigant. Rapid detection of phosphine resistance level is a prime key to implement an appropriate strategy for control the stored-product pests. Dihydrolipoamide dehydrogenase (DLD) is a key metabolic enzyme mediating the phosphine resistance in population of R. dominica, Tribolium castaneum and Caenorhabditis elegans. Analysis of the DLD sequences deposited in GenBank revealed that the P45/49S mutation was the most common one in many PH3-resistant stored-product pest insects. This information now enables direct detection of resistance using molecular diagnosis in field populations. We herein propose a method for rapid detection of phosphine resistance in R. dominica according to P49S point mutation of the DLD gene. Our data provides evidence that the ARMS-PCR method can be used for early warning of phosphine resistance in R. dominica in field conditions.

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Veröffentlicht

2018-11-09

Ausgabe

Nr. 463 (2018): Proceedings of the 12th International Working Conference on Stored Product Protection (IWCSPP) in Berlin, Germany, October 7-11, 2018

Rubrik

Session 9: Integrated Pest and Resistance Management

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