Effects of methoprene on extreme temperature tolerance and reproduction of <i>Tribolium castaneum</i> (Coleoptera: Tenebrionidae)

  • L. K. W. Wijayaratne Department of Entomology, University of Manitoba, 12 Dafoe Road, Winnipeg, Manitoba, Canada R3T 2N2
  • P. G. Fields Cereal Research Centre, Agriculture and Agri-Food Canada, Winnipeg, Manitoba, Canada R3T 2M9. Email: paul.fields@agr.gc.ca

Abstract

The juvenile hormone analogue methoprene is a reduced-risk insecticide. It disrupts insect development of immature stages preventing the emergence of adults. Several studies have shown that lower concentrations that permit the emergence of adults also have sub-lethal effects. Exposure to methoprene (Diacon II) at 3.33 ppm reduced the heat tolerance of Tribolium castaneum (Herbst) adults. However, it did not affect the heat tolerance of larvae at 0.07 ppm. Higher concentrations of methoprene were lethal to larvae without heat treatment. Methoprene (67 ppm) had no effect on the cold tolerance of adults. Furthermore, methoprene (0.03 ppm) did not alter cold tolerance of larvae. Exposure to 15°C for 2 weeks increased the cold tolerance of adults from 4 d to 7 d, and larvae 3 d to 5 d; however, methoprene concentrations had no effect on cold tolerance. Tribolium castaneum larvae exposed to methoprene (0.001 ppm) had lower fecundity as adults. Males were more affected than females in reducing the offspring when paired with untreated mates. These results show the potential of methoprene as an emerging insecticide and a viable alternative to currently used synthetic insecticides. The data on the effect of methoprene on extreme temperature tolerance of T. castaneum have been submitted to the Journal of Stored Products Research.

Keywords: Methoprene, Extreme temperature tolerance, Reproduction, Larvae, Adults
Veröffentlicht
2010-09-20
Rubrik
Section: Residual Insecticides - Synthetic and Botanical