Intra and interspecific variation assessment in Psocoptera using near spectoscopy

  • S. M. N. Lazzari Department of Zoology, Universidade Federal do Paraná, Caixa Postal 19020 – 81531-980 Curitiba, Paraná, Brazil, Email: lazzari@ufpr.br
  • F. C. Ceruti Department of Zoology, Universidade Federal do Paraná, Caixa Postal 19020 – 81531-980 Curitiba, Paraná
  • J. I. Rodriguez-Fernandez Department of Zoology, Universidade Federal do Paraná, Caixa Postal 19020 – 81531-980 Curitiba, Paraná; Colección Boliviana de Fauna, La Paz, Bolivia.
  • G. Opit Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA.
  • F. A. Lazzari Department of Zoology, Universidade Federal do Paraná, Caixa Postal 19020 – 81531-980 Curitiba, Paraná

Abstract

Several species of Psocoptera are associated with and damage grains and other stored products, books, historical documents, and insect collections. Their small size and lack of expressive morphological variation make it a difficult group for species identification. The spectra of adult males and females of 10 psocid species from the genus Liposcelis were obtained by near infrared spectroscopy (NIRS) and analyzed. Each specimen was placed on a diffuse reflectance accessory of a NIR spectrometer to obtain the respective spectrum, using ten replicates for each species or sex. All spectra were analyzed by combined methods of multivariate analysis using the technique of crossed validation for the multivariate models. The analysis discriminated the species without significant overlapping among the species spectral patterns. The NIRS also revealed variation in the metabolomic profile of males and females; however, it is still possible to distinguish the species using only males or females or even from mixed sex samples. NIRS technique proved to be a powerful tool to discriminate species both at intra and interspecific levels based on dispersion spectral patterns of individual specimens.

Keywords: Biological systems, Liposcelididae, stored product pests, Vibrational spectroscopy.

Veröffentlicht
2010-09-02