Insecticidal effect of anisaldehyde against <i>Acanthoscelides obtectus</i> and <i>Callosobruchus maculatus</i> (Coleoptera: Bruchidae)

  • A. F. Ndomo University of Dschang, Department of Chemistry, Faculty of Science, Laboratory of Applied and Environmental Chemistry, P.O. Box 183, Dschang, Cameroon
  • L. A. Tapondjou University of Dschang, Department of Chemistry, Faculty of Science, Laboratory of Applied and Environmental Chemistry, P.O. Box 183, Dschang, Cameroon. Email tapondjou2001@yahoo.fr
  • F. M. Tchouanguep University of Dschang, Faculty of Science, Department of Biochemistry, Laboratory of Medicinal plants, Science and Nutrition, P.O. Box 67, Dschang, Cameroon

Abstract

In the present study, anisaldehyde, a compound found in the essential oil of Clausena anisum-olens, was tested for its insecticidal activities against Acanthoscelides obtectus and Callosobruchus maculatus. The amounts of anisaldehyde applied were 0, 0.5, 1, 2 and 4 μL diluted in 1mL of acetone and applied to 40 g of either beans or cowpeas corresponding to the doses of 0, 0.008, 0.016, 0.033 and 0.066 μL/g of seed. Additionally, adsorbent clay was used as a carrier of this product in order to increase the persistence of its insecticidal activity over time. This clay was mixed with the aforementioned volumes of anisaldehyde to form a powder formulation. Furthermore, to assess the insecticidal effect over time, the F1 progeny production was also evaluated. These two products caused significant mortality in the two tested insects. Nevertheless, C. maculatus was more susceptible than A. obtectus at tested doses. The progeny production decreased with the increasing doses of anisaldehyde and ACP with 0 % at the highest dose (0.066 μL/g). According to the LD50, LD95 and their confidence intervals, the toxicity of ACP was significantly different (P < 0.05) to anisaldehyde at the tested doses towards A. obtectus adults. However, there was no significant difference observed between the effects of these two products towards C. maculatus. These preliminary results suggest that anisaldehyde and ACP could be used in stored-product protection, but this needs further research. Research is also needed to determine its toxicity on rats in order to assess its potential hazards for workers and consumers.

Keywords: Anisaldehyde, Clay, Contact toxicity, Bruchids
Veröffentlicht
2010-09-02