HPLC analysis of anthocyanins and flavonols and expressions of different copies of <i>F3´5´H</i> in grapevine transgenic lines

Autor/innen

  • Maryam Pezhmanmehr Department of Horticulture, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • Amir Moosavi National Institute of Genetic Engineering and Biotechnology (NIGEB), P.O. Box 14155-6343, Tehran, Iran
  • Ali Ebadi Department of Horticultural and Landscape Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
  • Debra McDavid CSIRO Plant Industries, Adelaide, PO Box 350, Glen Osmond, SA 5064, Australia
  • Amanda R. Walker CSIRO Plant Industries, Adelaide, PO Box 350, Glen Osmond, SA 5064, Australia

DOI:

https://doi.org/10.5073/jka.2016.453.031

Schlagworte:

Vitis vinifera, anthocyanin, flavonol, F3´5´H, silencing

Abstract

Transgenic lines of grapevine containing silencing constructs of F3´5´H based on ihpRNA mechanism were studied for their flavonoids and for the expression of F3´5´H gene copies. 83-89 % of transgenic lines showed various degrees of silencing, and increase of di-hydroxylated anthocyanins and kaempferol were observed in the leaves of transgenic lines.

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Veröffentlicht

2016-07-26