Laboratory evaluation of diatomaceous earth against main stored product insects

Abstract

The sensitivity of the main external and internal stored product insect pests to the commercial formulation of Detia Degesch Diatomaceous Earth – DDDE - Inerto (DE) was studied in laboratory experiments. The tested insects were adults of internal feeders Sitophilus oryzae Rhyzopertha dominica and external feeders Oryzaephilus surinamensis, Tribolium castaneum, and larvae (third instar) of T.castaneum. The DE was applied to wheat grain of 12% moisture content at concentrations of 0.5, 1.0, 2.0 and 4.0 g/kg of grain. The treated and untreated (control) grain were kept at 28°C and 65 ± 5% r.h. The numbers of dead and survived insects were counted two, three and four weeks after treatment. The number of adult progeny was counted nine weeks after treatment. At a concentration of 0.5 g/kg, mortality of S. oryzae and O. surinamensis after three weeks of exposure to DE were 92 and 86%, respectively. In contrast, mortality of T. castaneum and R. dominica adults was 3 and 37%, respectively. Progeny production of O. surinamensis and T. castaneum at a concentration of 2 g/kg was negligible, since only few individuals were recorded nine weeks after treatment, in comparison with the high progeny production in the control grain. The progeny of S. oryzae was also reduced. In contrast, for R. dominica was reduced only twice, in comparison with the control. In the case of T. castaneum larvae, at a concentration of 2 g/kg, after 4 weeks of exposure, 37% of the larvae emerged to adults, compared with 95% in control. Nine weeks after treatment, the number of F₁adults was 100% suppressed. DE efficacy was similar at 4 g/kg. Based on the findings of the present study, the efficacy of the tested DE was influenced by DE concentration, insect species, developmental stage and exposure interval to the treated commodity.

Keywords: Diatomaceous earth, Stored product insects, Wheat grain