Aspects of the methodology validation for light filth in fruit pulp

Abstract

Methodology validation is an important tool to support the activities of metrology in the quantification analysis. Extraneous materials may be present in the pulps due to the conditions or the practices of production, storage and distribution. Since there is no specific methodology for fruit pulp light filth quantification a research has been carried out to test two methods for isolation of light filth in mango, strawberry, tomato and guava pulps. For guava, mango and tomato pulps the AOAC Official Method 964.23 was used, and for strawberry pulp AOAC Official Method 950.89, with adaptations. The micro-analytic standard for insect fragment and rodent hair was prepared in the laboratory. The study has been conducted on 63 samples of 100 g of pulp analyzed in duplicate after contamination with 5, 15, 30 insect fragments (IF) per 100 g and 5, 10, 15 rodent hair (RH) per 100 g, with blank samples as a control. The validation parameters used were precision and accuracy. Recovery of insect fragment was considered stisfactory in mango, tomato, guava pulps, and the average recovery ranged from 87 to 96%. For strawberry pulp, the recovery rate ranged from 68 to 80%. For rodent hairs, the best recovery rate was observed in mango pulp ranging from 73 to 81%, followed by guava pulp ranging from 65 to 76%, tomato pulp ranging from 50 to 67%, and strawberry pulp ranging from 33 to 35%. It was concluded that the methodologies used to detect light filth in guava, mango, tomato and strawberry pulps can be adopted in the monitoring routine of fruit pulp extraneous material contamination even though they were found not very efficient for rodent hairs.

Keywords: Insect fragment, Rodent hair, AOAC determination method, Recovery rate, Fruit pulp.