Discrimination of fennel chemotypes applying IR and Raman spectroscopy – discovery of a new <i>γ</i>-asarone chemotype

Autor/innen

  • Andrea Krähmer Julius Kühn-Institute, Federeal Research Center for Cultivated Plants, Institute for ecological Chemistry, Plant Analysis and Stored Product Protection, Königin-Luise-Str. 19, D-14195 Berlin, Germany
  • Gennadi Gudi Julius Kühn-Institute, Federeal Research Center for Cultivated Plants, Institute for ecological Chemistry, Plant Analysis and Stored Product Protection, Königin-Luise-Str. 19, D-14195 Berlin, Germany
  • Hans Krüger Julius Kühn-Institute, Federeal Research Center for Cultivated Plants, Institute for ecological Chemistry, Plant Analysis and Stored Product Protection, Erwin-Baur-Str. 27, D-06484 Quedlinburg, Germany
  • Lothar Henning University of Leipzig, Department of Organic Chemistry, Johannisallee 29, D-04103 Leipzig, Germany
  • hartwig Schulz Julius Kühn-Institute, Federeal Research Center for Cultivated Plants, Institute for ecological Chemistry, Plant Analysis and Stored Product Protection, Königin-Luise-Str. 19, D-14195 Berlin, Germany

DOI:

https://doi.org/10.5073/jka.2016.453.051

Schlagworte:

ATR-FTIR, FT-Raman, NIRS, Foeniculum, chemometry, γ-asarone

Abstract

Various vibrational spectroscopy methods have been applied to classify different fennel chemotypes according to their individual profile of volatile substances.
Intact fennel fruits of different chemotypes could be successfully discriminated by Attenuated Total Reflectance Fourier transform Infrared (ATR-FTIR) and Near Infrared (NIR) spectroscopy. Solvent extracts (CCl4) of the considered fennel fruits showed characteristic fingerprints with marker bands related to the individual volatile components (trans-anethole, fenchone, estragole, piperitenone oxide, γ-asarone, limonene) for ATR-FTIR and FT-Raman spectroscopy.
Especially νC=C and νC=O absorption bands contribute to the different spectral profiles. Based on hierarchical cluster analysis, the considered fennel accessions were classified according to gas chromatographic (GC) and vibrational spectroscopic data. Furthermore, even a discrimination of “sweet” and “bitter” fennel fruits, both belonging to the trans-anethole chemotype, could be successfully performed. All vibrational spectroscopical techniques used in this study are rapid and easy to apply. Hence, they allow different fennel chemotypes to be reliably distinguished and can also be used for on-site measurement in free nature.

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Veröffentlicht

2016-07-26