The effect of vineyard long-term monoculture soil on production of volatile compounds and photosynthetic apparatus in grapevine leaves

Abstract

The effect of soil collected from long-term (1007 years of duration of monoculture) and short-term (55 years) grapevine monoculture on production of volatile organic compounds (VOC’s) and on functional parameters of photosystem II in grapevine leaves was analyzed. Grapevine plantlets grown in tested soils showed differences in VOC’s production after five months cultivation. Chlorophyll a fluorescence measurements by JIP-test revealed that the photosystem II was less efficient but the fluorescence intensity increased in plant growing in soil from the long-term monoculture compared to plants growing in the short term monoculture soil. Pseudomonas spp. carrying the biocontrol genes phlD and hcnAB were isolated from long-term monoculture soil. A consortium of ten of these isolates was added to the short term monoculture soil. The plants grown in this inoculated soil showed similar changes in fluorescence intensity and photosystem efficacy as the plants growing in long term monoculture. In this study, simple tools for measurement of a “soil effect” by measuring only a leaf have been tested successfully. They have allowed exhibiting the influence of long-term monoculture on plant physiology.