Analysis of red wine phenolics: Comparison of HPLC and spectrophotometric methods

Authors

  • U. Vrhovsek
  • F. Mattivi
  • A. L. Waterhouse

DOI:

https://doi.org/10.5073/vitis.2001.40.87-91

Keywords:

polymeric polyphenols, spectrophotometric methods, HPLC

Abstract

A recently developed ion-pair normal phase HPLC method which allows a precise chromatographic evaluation of the whole class of high-molecular-mass phenolics of wine was used in order to check the performance of spectrophotometric methods. Thirty-two monovarietal red wines (vintages 1993 and 1998) were analysed for total high-molecular-mass phenolics, proanthocyanidins with 2-4 units, and proanthocyanidins formed by 5 or more units, by means of the normal phase HPLC method. In addition the following spectrophotometric assays were performed: total phenols by Folin-Ciocalteu, Bate-Smith transformation of proanthocyanidins into cyanidin and catechins and proanthocyanidins reactive to vanillin.

 

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Published

2015-04-30

Issue

Vol. 40 No. 2 (2001): Vitis

Section

Article

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