Identification and typing of grapevine phytoplasma amplified by graft transmission to periwinkle

Authors

  • Edna Tanne
  • Sigalit Orenstein

DOI:

https://doi.org/10.5073/vitis.1997.36.35-38

Keywords:

phytoplasma, grapevine yellows, periwinkle, PCR, restricted PCR

Abstract

Yellows of grapevine are spreading in many parts of the world and have been attributed to mycoplasma-libe organisms (MLOs; phytoplasma). A classification and basic understanding of grapevine phytoplasma requires samples of sufficient size which are not easy to prepare. Various phytoplasma isolates have been graft-transmitted from grapevine to periwinkle. Using PCR we show that the various phytoplasma types found in grafted periwinkle faithfully match the type found in the donor grapevine. PCR analysis of phytoplasma in periwinkle was more pronounced than the respective analysis in the donor grapevine. Therefore transmission to periwinkle may facilitate the study of grapevine phytoplasma.

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Published

2015-08-06

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