Grapevine fanleaf virus detection in various grapevine organs using polyclonal and monoclonal antibodies

Authors

  • Brigitte Huss
  • B. Walter
  • L. Etienne
  • M. H. V. van Regenmortel

DOI:

https://doi.org/10.5073/vitis.1986.25.178-188

Keywords:

fanleaf, virus, analysis, serology, leaf, shoot, root

Abstract

Monoclonal antibodies prepared with grapevine fanleaf virus (GFV) are useful for detecting the virus in plant extracts. In this paper we describe comparisons between different ELISA techniques using rabbit and chicken immunoglobulins as weil as monoclonal antibodies (MCA). The technique using chicken immunoglobulins for coating the plates followed by MCA and goat anti-mouse phosphatase conjugate was the best one for detecting GFV in plant sap. In this technique, ascitic fluids containing MCA could be diluted up to 10-6.
Our experiments clearly demonstrate that the detection of GFV is possible in grapevine not only from leaves or rootlets, but also from wood shavings, without grinding them. We replaced the classical nicotine containing extraction medium by a harmless phosphate or Tris-HCl buffer. To detect GFV with these media it is essential that the buffer should contain polyvinylpyrrolidone and that its molarity should not be less than 0.1 M.

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Published

2015-12-17

Issue

Vol. 25 No. 3 (1986): VITIS

Section

Article

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