High resolution mapping and identification of candidate genes for the BaMMV/BaYMV-resistance gene rym13 and Ryd3 involved in BYDV-tolerance of barley
DOI:
https://doi.org/10.5073/dissjki.2019.004Abstract
Barley yellow mosaic disease, caused by Barley mild mosaic virus (BaMMV) and Barley yellow mosaic virus (BaYMV), and barley yellow dwarf, caused by Barley yellow dwarf virus (BYDV), are important diseases of barley causing high yield losses. As BaMMV and BaYMV are transmitted by the soil-borne plasmodiophorid Polymyxa graminis and BYDV by aphids, the only environmently sound way to prevent these yield losses is breeding for resistant/tolerant cultivars.
In this thesis, high resolution mapping and candidate gene analysis for the BaMMV/BaYMV resistance gene rym13 and the BYDV tolerance conferring gene Ryd3 was conducted. For rym13, a high resolution mapping population based on 5,191 F2 plants was established and marker saturation was conducted by mapping the flanking markers to published genomic resources of barley. Using these genomic resources in combination with next-generation-sequencing-techniques led to a fast saturation of the resistance harbouring interval and identification of candidate genes. Analysing phenotypic and genotypic data revealed an independent inheritance of the BaMMV and BaYMV resistance in the cultivar ‘Taihoku A’. Two candidate genes for rym13, co-segregating with BaMMV resistance in an independent population, were identified.
Regarding Ryd3, the former mapping population established by Lüpken et al. (2014) was extended to 7,427 F2 plants. After mapping the co-segregating markers to the published reference genome of barley, the interval related to tolerance was shortened to 104.14 mio bp. This interval harbours a number of candidate genes. For further investigations to unravel the mechanism of tolerance, a TILLING population was established in the course of this thesis.
This study lays the foundation for subsequent isolation and validation of rym13 and Ryd3.
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