Identifikation des Mehltauresistenzlocus <em>Rpv10</em> für die Rebenzüchtung

Authors

  • Florian Schwander Institute for Grapevine Breeding

Abstract

Rpv10, a new locus originating from the Asian wild species Vitis amurensis mediates resistance against downy mildew on grapes caused by Plasmopara viticola. This locus was newly identified using a population derived from a cross between grapevine breeding strain Gf.Ga-52-42 and cultivar ‘Solaris’ consisting of 265 F1-individuals. A genetic map of the cross-population was constructed using SSR-Markers and all individuals were screened for downy mildew resistance. Quantitative trait locus (QTL) analysis revealed two major QTL on linkage groups (LGs) 09 and 18. ‘Solaris’ inherited the resistance related locus on LG 09 explaining up to 50 % of the phenotypic variation in the population. This locus, named Rpv10, was further restricted by adding newly developed locus-specific SSR markers. A minimum one-LOD confidence interval of 0.5 cM was achieved in MQM analyses. The region within the flanking markers spans a distance of 1.6 cM corresponding to 79 kb in the 12x reference genome sequence of PN40024. One resistance gene analogue (RGA) of the NBS-LRR type, an ethylene-responsive transcription factor, an ankyrin-like protein and a probable ribonuclease are annotated within this range to be considered as putative candidates for mediating resistance. The major QTL on LG 18 transmitted by Gf.Ga-52-42 is identical to the Rpv3 locus known from ‘Regent’ and ‘Bianca’. The F1 sub-population which contains the Rpv3 as well as the Rpv10 locus showed a significantly higher degree of resistance indicating additive effects by pyramiding of resistance loci. Furthermore, the marker data revealed ‘Severnyi’ as the descent responsible for introgression of the V. amurensis resistance in ‘Solaris’ contradicting the previously assumed derivation. Genetic resources having a V. amurensis background were screened for the Rpv10 genotype. Twenty-two out of ninety-four varieties were identified providing the Rpv10 locus which can potentially be used for implementing the locus into the further breeding progress. This screening also revealed the most suitable SSR-markers for a reliable identification of the Rpv10 locus in marker assisted breeding (MAS).

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Published

2012-03-26

Issue

2012

Section

Dissertation

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