Untersuchungen zur Resistenz von Pelargonien gegen die Bakterien <em>Xanthomonas hortorum</em> (pv. <em>pelargonii</em>) und <em>Ralstonia olanacearum</em>...

...sowie Etablierung von biotechnologischen und molekularen Methoden zur züchterischen Nutzung derartiger Resistenzmerkmale

Authors

  • Josefine Engel Institute for Resistance Research and Stress Tolerance, Institute for Breeding Research on Horticultural and Fruit Crops

Abstract

Pelargonium  is  one  of  the  most  important  ornamental  plants  in  home  and  garden.  Cultivars are propagated vegetatively. The two bacterial pathogens Xanthomonas hortorum pv. pelargonii (Xhp) and Ralstonia solanacearum (Rs) cause bacterial wilt and blight and result in high economic losses. The characteristic symptoms of both diseases are wilting leaves. Because the infection pathway for the two pathogens is different, one aim of this study was the development of a reliable inoculation method for both pathogens.
1. The screening methods were developed on plant material provided by Elsner pac® Dresden. 114 species of different sections were tested. Based on this inoculation methods 3 genotypes with resistance against Xanthomonas hortorum pv. pelargonii (Xhp) and Ralstonia solanacearum (Rs) were identified.
2. Studies were realized to transfer the resistance, which was detected in a wild species to P. x hortorum. As sexuell crossings cannot be achieved protoplast fusions were conducted by developing and adapting respective protocols.
3. The protoplast fusions were realized chemically (with Polyethylenglycol) and electrophysically.
4. As a result 320 plants could be regenerated by improving the protocoll. 43 plants were phenotypically interesting based on their habitus and their leave structure. 277 plants equate the wild type phenotypically.
5. For the regeneration some additives like Chitosan and Nitroprussid were testet in regard to the influence on the growth of the calli.
The  regenerated  plants  were  cultivated  in  the  greenhouse  and  characterized  phenotypically, molecularly and with GISH and Flowcytometry. Besides  this,  volatile  ingredients  were  determined.  Until  now  all  regenerated  plants  derived from homofusionates of the wild type.

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Published

2013-10-11

Issue

2013

Section

Dissertation

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