Züchtungsmethoden der nächsten Generation für Kamille: Bewertung der genetischen Diversität, der Ploidievariation und Identifizierung von Marker-Merkmal Assoziationen

  • Lars-Gernot Otto Quantitative Genetics Research Group, Department Plant Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Seeland OT Gatersleben, Germany
  • Jonathan Brassac Quantitative Genetics Research Group, Department Plant Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Seeland OT Gatersleben, Germany
  • Prodyut Mondal Research Group of Pharmaceutical Biotechnology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
  • Marlies Sonnenschein Pharmaplant GmbH, Artern, Germany
  • Bartolome Plocharski Pharmaplant GmbH, Artern, Germany
  • Wolfram Junghanns Dr. Junghanns GmbH, Aschersleben, Germany
  • Susanne Preis Research Group of Pharmaceutical Biotechnology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
  • Jörg Degenhardt Research Group of Pharmaceutical Biotechnology, Martin-Luther University Halle-Wittenberg, Halle (Saale), Germany
  • Mariateresa Lazzaro Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy
  • Marika Bocchini Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy
  • Emidio Albertini Department of Agricultural, Food and Environmental Sciences, University of Perugia, Perugia, Italy
  • Andreas Plescher Pharmaplant GmbH, Artern, Germany
  • Beate Fraust Quantitative Genetics Research Group, Department Plant Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Seeland OT Gatersleben, Germany
  • Sang He Quantitative Genetics Research Group, Department Plant Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Seeland OT Gatersleben, Germany
  • Jochen Reif Quantitative Genetics Research Group, Department Plant Breeding Research, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraße 3, D-06466 Seeland OT Gatersleben, Germany
  • Timothy Sharbel Global Institute for Food Security, University of Saskatchewan, Saskatoon, Canada
Schlagworte: Matricaria recutita, chamomile, genetic diversity, GBS, AFLP

Abstract

Chamomile (Matricaria recutita L.) has a long history of use in herbal medicine with various applications, and the flower heads contain numerous medicinally active compounds. Next generation sequencing (NGS) approaches are applied to exploit genetic resources in the major crop plants to develop genomic resources, and to enhance breeding. Genotyping-by-sequencing (GBS) has been used to evaluate the genetic structure of cultivated populations in the non-model crop chamomile using 6495 SNP markers, and to perform a genome wide association study (GWAS) identifying sequences significantly associated with the medicinally important alpha-bisabolol content. Ploidy variation in chamomile was investigated by high-throughput flow-cytometry. Di-, tri- and tetraploid plants were identified, and in field trials characterized. Since seeds are not needed in the harvested product of chamomile, triploidy could be a way to obtain a sterile chamomile variety, omitting the problems of chamomile seeds lying up to 15 years dormant in the soil and facilitating crop rotation in the fields.

Veröffentlicht
2018-12-20
Rubrik
Themenkreis E: Wildsammlung, Inkulturnahme, Züchtung