Higher TIER bumble bees and solitary bees recommendations for a semi-field experimental design

  • Silvio Knäbe Eurofins Agroscience Services Ecotox GmbH
  • Matthew J. Allan Atlantic Pollination Ltd., 18 North Street, Glenrothes, KY7 5NA, United Kingdom
  • Annika Alscher Rifcon GmbH, Hirschberg
  • Kristin Amsel BioChem agrar GmbH, Kupferstraße 6, 04827 Machern/OT Gerichshain, Germany
  • Christian Classen Rifcon GmbH, Hirschberg
  • Magdaléna Cornement Innovative Environmental Services (IES) Ltd, Benkenstrasse 260, 4108 Witterswil, Switzerland
  • Charlotte Elston Syngenta Ltd, Jealott’s Hill International Research Centre Bracknell, Berkshire, RG42 6EY, United Kingdom
  • Nina Exeler Bayer CropScience, 40789 Monheim, Germany
  • Lea Franke Eurofins Agroscience Services Ecotox GmbH
  • Malte Frommberger Julius Kühn-Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Bienenschutz
  • Hervé Giffard Testapi, 464 Sarré, Gennes, 49350 Gennes-Val-de-Loire, France
  • Juan Sorlí Guerola Trialcamp SLU, Avda. Antic Regne de Valencia 25, 46290 Alcasser, Spain
  • Sabine Hecht-Rost Rifcon GmbH, Hirschberg
  • Bettina Hodapp Innovative Environmental Services (IES) Ltd, Benkenstrasse 260, 4108 Witterswil, Switzerland
  • Ines Hotopp tier3 solutions, Kolberger Str. 61-63, 51381 Leverkusen, Germany
  • Carole Jenkins Envigo, Woolley Road, Alconbury, Huntingdon, Cambridgeshire PE28 4HS, United Kingdom
  • Tobias Jütte Julius Kühn-Institut, Bundesforschungsinstitut für Kulturpflanzen, Institut für Bienenschutz
  • Stefan Kimmel Innovative Environmental Services (IES) Ltd, Benkenstrasse 260, 4108 Witterswil, Switzerland
  • Olaf Klein Eurofins Agroscience Services Ecotox GmbH
  • Britta Kullmann tier3 solutions, Kolberger Str. 61-63, 51381 Leverkusen, Germany
  • Johannes Lückmann Rifcon GmbH, Hirschberg
  • Markus Persigehl tier3 solutions, Kolberger Str. 61-63, 51381 Leverkusen, Germany
  • Ivo Roessink Wageningen Environmental Research, Droevendaalsesteeg 4, 6708 PB Wageningen, The Netherlands
  • Christof Schneider BASF, 67117 Limburgerhof, Germany
  • Alexander Schnurr BioChem agrar GmbH, Kupferstraße 6, 04827 Machern/OT Gerichshain, Germany
  • Verena Tänzler IBACON GmbH, Arheilger Weg 17, 64380 Roßdorf, Germany
  • Jozef J. M. van der Steen Alveus AB Consultancy, Oisterwijk, The Netherlands
Schlagworte: semi-field testing, non-Apis bees, bumble bees, solitary bees

Abstract

The publication of the proposed EFSA risk assessment guidance document of plant protection products for pollinators highlighted that there are no study designs for non-Apis pollinators available. Since no official guidelines exist for semi-field testing at present, protocols were proposed by the ICPPR non-Apis working group and two years of ring-testing were conducted in 2016 and 2017 to develop a general test set-up. The ringtest design was based on the draft EFSA guidance document, OEPP/EPPO Guideline No. 170 and results of discussions regarding testing solitary bees and bumble bees during the meetings of the ICPPR non-Apis workgroup.
Ring-tests were conducted with two different test organisms, one representative of a social bumble bee species (Bombus terrestris L; Hymenoptera, Apidae) and one representative of a solitary bee species (Osmia bicornis L; Hymenoptera, Megachilidae). The species are common species in Europe, commercially available and widely used for pollination services. Several laboratories participated in the higher-tier ring tests. 15 semi-field tests were conducted with bumble bees and 16 semi-field tests were done with solitary bees in 2016 and 2017.
Two treatment groups were always included in the ringtests: an untreated control (water treated) and the treatment with dimethoate as a toxic reference item (optional other i.e. brood-affecting substances fenoxycarb or diflubenzuron). The toxic reference items were chosen based on their mode of action and long term experience in honey bee testing.
A summary of the ringtest results will be given and the recommendations for the two semi-field test designs will be presented.

Veröffentlicht
2020-05-11