Worldwide diffusion of Fig latent virus 1 in fig accessions and its detection by serological and molecular tools

Autor/innen

  • G. Gattoni
  • A. Minafra
  • M. A. Castellano
  • A. De Stradis
  • D. Boscia
  • T. Elbeaino
  • M. Digiaro
  • G. P. Martelli

Abstract

A virus with filamentous particles ca. 700 nm long, denoted Fig latent virus 1 (FLV-1) is widespread in Apulian (southern Italy) fig orchards, in trees showing or not mosaic symptoms and in symptomless seedlings. The virus was transmitted by sap inoculation to a very restricted range of herbaceous hosts without inducing apparent symptoms and was transmitted through fig seeds to a very high percentage (80 to 100 %). It was successfully purified from root tissues of infected figs. A virus-specific antiserum raised in rabbits, proved useful for its detection in fig leaf dips by immunosorbent electron microscopy (ISEM), Western Blot, dot immuno-binding (DIBA), ELISA. The viral genome structure resembles that of members of the genus Trichovirus in the family Flexiviridae.

Keywords: fig latent virus, Trichovirus, serology, ISEM, Western blot, DIBA, ELISA

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Veröffentlicht

2010-09-28