<i>In vitro</i> screening of interspecific hybrids (<i>Malus</i> spp.) for resistance to apple proliferation

Autor/innen

  • C. Bisognin
  • A. M. Ciccotti
  • A. Salvadori
  • W. Jarausch
  • M. S. Grando

Abstract

A breeding programme was set up six years ago in Trentino as part of the Project “Scopazzi del Melo - Apple Proliferation” (SMAP) in order to obtain AP resistant apple rootstocks.

Twenty-six hybrids generated from the crossings (Malus sieboldii, second generation, x Malus domestica) were micropropagated and studied in standardised conditions. An in vitro screening system for AP resistance previously set for the parents of the crosses was adopted and modified. Specific symptoms of the disease, as well as height and basal proliferation of the shoot and size of the leaves, were recorded in vitro at 3 months post-inoculation. At the same time, phytoplasma concentration was determined in the whole shoot by quantitative RT-PCR. An in vitro disease index taking into account all the above-mentioned parameters was developed.

Each healthy genotype was graft-inoculated in triplicate with two different phytoplasma strains after plant rooting and acclimatisation. Phenotype and phytoplasma titre were evaluated in the roots the year after infection.

Preliminary results indicated that the resistance trait segregates in the progenies. The resistant genotypes had lower phytoplasma concentrations than the susceptible controls, did not show AP-specific symptoms and their growth was not affected by infection. By comparing the resistant behaviour of the same genotypes, the in vitro screening allows for a quick selection of genotypes that are worth evaluating in the field for agronomic traits.

Keywords: Apple Proliferation, Malus sieboldii, resistance screening, quantitative real-time PCR, disease index

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Veröffentlicht

2010-09-29