Study on the insecticidal activity compounds of the essential oil from <i>Syzygium aromaticum</i> against stored grain insect pests

  • L. Zeng Guangdong Institute for Cereal Science Research, No.222 North Yuexiu Road, Guangzhou, Guangdong, 510050, P. R. China. Email: zengl@tom.com
  • C. Z. Lao Guangdong Institute for Cereal Science Research, No.222 North Yuexiu Road, Guangzhou, Guangdong, 510050, P. R. China
  • Y. J. Cen Laboratory of Insect Ecology, South China Agricultural University, Wusan Road, Guangzhou, Guangdong, 510640, P. R. China
  • L. Zeng Laboratory of Insect Ecology, South China Agricultural University, Wusan Road, Guangzhou, Guangdong, 510640, P. R. China
  • G. W. Liang Laboratory of Insect Ecology, South China Agricultural University, Wusan Road, Guangzhou, Guangdong, 510640, P. R. China

Abstract

Insect pests are a major cause of damage in stored grain around the world. To control the stored grain insects, synthetic insecticides have been used extensively for many years, resulting in insect populations that are resistant to insecticides. Consequently there is an interest to find alternatives to chemical pesticides. The essential oil from Syzygium aromaticum (clove oil) has a number of bioactive compounds. The chemical constituents of the clove oil were analyzed by GC-MS, and 9 of 18 compounds were identified. The main compound (83%) was 2-methoxy-4-(2-propenyl)-phenol the second most common compound (12%) was trans-caryophyllene. These two pure compounds and clove oil were tested for toxicity and repellency against Rhyzopertha dominica, Sitophilus oryzae and Tribolium castaneum. The pure compounds were tested at the dosages found in clove oil. The mortality from 2-methoxy-4-(2-propenyl)-phenol was not significantly different from clove oil, suggesting that the activity of clove oil was solely due to this major compound. The repellency results were more complex. 2-methoxy-4-(2-propenyl)-phenol was more repellant than clove oil. Trans-caryophyllene was less toxic and less repellant than both clove oil and 2-methoxy-4-(2-propenyl)- phenol. The potential for these compounds to be used to control stored product insects is discussed.

Keywords: Essential oils, Syzygium aromaticum, Clove oil, Insecticidal activity compounds, Stored grain insects
Veröffentlicht
2010-09-02