An investigation on Rose Mosaic Disease of Rose in Hatay-Turkey

Abstract

Field inspections were carried out to investigate Apple mosaic virus (ApMV), Arabis mosaic virus (ArMV) and Prunus necrotic ringspot virus (PNRSV) which are associated with rose mosaic disease (RMD) during the years of 2008 and 2009. Characteristic symptoms, including chlorotic line patterns (zigzag pattern), vein-banding and mottles in leaves were observed during spring. Symptoms were also evident during summer on leaves produced until early summer. Flower abnormalities as phyllody were also exhibited during autumn. Distortion and reduction in flower size and early leaf drop have been observed on symptomatic plants in winter. Leaf samples taken from 15 rose plants from ‘Rosa hybrida L.’ neighboring stone fruit orchards were tested by mechanical inoculation to herbaceous plants and enzymelinked immunosorbent assay (DAS-ELISA) for the presence of ApMV, ArMV and PNRSV, which are the viruses related to RMD. Catharanthus roseus L. G. Don, Chenopodium amaranthicolor Coste and Reyn., C. quinoa Wild, Cucumis sativus L., Gomphrena globosa L., Lagenaria siceraria (Mol.) Standl, Nicotiana benthamiana L., N. clevelandii L., Nicotiana glutinosa L., Phaseolus vulgaris L., Vigna unguiculata L. test plants were incubated after mechanical inoculation for symptom appearance at 25°C±2 and 16:8 h photoperiod (day:night) conditions in an insectproof room. Symptoms including chlorotic local lesions, systemic necrosis, stunting and yellow mottling began to appear on C. quinoa and C. sativus in 2-3 weeks after sap inoculation. Serological tests of test plants are in progress. The rose plants showing symptoms in home gardens were re-tested for the viruses in spring by ELISA. According to the results of the Bioassay by sap inoculation and ELISA on symptomatic rose plants, the causal agent of RMD is PNRSV. The viruses affecting rose plants spread through cuttings from a diseased plant because new plants are generally produced by the rooting of cuttings in home gardens in Hatay. Further detailed investigations are necessary to find out the causal agent/s of RMD in rose in the region, because infected rose plants can be an important factor in the epidemiology of virus diseases caused by these agents in rose plantations.

Keywords: ApMV, Bioassay, ELISA, Oil Rose, PNRSV, virus