Stem starch reserves studied by on-solid reactions coupled with reflectance detections in water stressed grapevines

  • L. Rustioni DISAA – Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, Milano, Italy
  • J. C. Herrera Division of Viticulture and Pomology, Department of Crop Sciences, University of Natural Resources and Life Sciences Vienna, Tulln, Austria
  • O. Failla DISAA – Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Milano, Milano, Italy
  • E. Peterlunger Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy
  • P. Sivilotti Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy
Keywords: drought; stain; wood reserves; starch-iodine complexation; water potential; non-structural carbohydrates.

Abstract

Wine grape is usually grown under water deficit conditions that could negatively impact plant reserves, including the organic carbon storage in perennial organs such as woody stems. Assessing the carbohydrate status in woody organs is therefore of interest as it can influence vegetative growth in the successive season. In this study, we aimed to apply an on-solid colour reaction (using Lugol's iodine solution) coupled with reflectance spectroscopy detection to assess the grapevine canes' starch accumulation in response to short drought periods. We used two Vitis vinifera cultivars ('Cabernet Sauvignon' and 'Syrah') that were subjected to three different water conditions (well-watered; early water stress; late water stress) during the growing season as case study. We sampled woody stem tissue during winter rest. The results showed that water stress reduced the starch storage in 'Syrah', especially when imposed late and recovery time was not enough for carbon restoration, while 'Cabernet Sauvignon' was not affected. The results showed that the sensitivity of the method used here is adequate to assess starch accumulation differences due to drought treatments in grapevine canes. Moreover, the analytical approach appears fast, low cost, and promising for future physiological and agronomical research applications.

Published
2019-01-28
Section
Article