A rapid and inexpensive RNA-extraction method for high-throughput virus detection in grapevine

  • N. Steinmetz Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Geilweilerhof, Siebeldingen, Germany
  • G. Michl Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Geilweilerhof, Siebeldingen, Germany
  • M. Maixner Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Geilweilerhof, Siebeldingen, Germany
  • C. Hoffmann Julius Kühn-Institute (JKI), Federal Research Centre for Cultivated Plants, Institute for Plant Protection in Fruit Crops and Viticulture, Geilweilerhof, Siebeldingen, Germany
Keywords: Vitis vinifera; virus diagnostic; modified silica-extraction.

Abstract

The extraction of RNA from grapevine tissue is a crucial step for virus diagnostics via multiplex reverse transcription-polymerase chain reaction (mRT-PCR). Conventional methods are either time-consuming or expensive when convenient extraction kits are used. Here we present an easy, but reliable extraction method that fulfills the requirements of epidemiological research (high sample throughput with maximum accuracy). A further advantage of the protocol beside the low costs is the absence of harmful chemicals like phenol or chloroform and the possibility to manage 'high-throughput' extractions and analyses.

Published
2020-02-13
Section
Article