Accumulated data and results from the recent study of dsRNA isolated from grapevines used in experiments of insect and graft transmission of 'Shiraz' disease

Authors

  • D. Goszczynski Plant Health and Protection, Agricultural Research Council, Queenswood, Pretoria, South Africa

DOI:

https://doi.org/10.5073/vitis.2020.59.111-116

Keywords:

'Shiraz' disease; graft and mealybug trans-mission; GVA variants; RT-PCR; HTS

Abstract

RT-PCR amplicons of dsRNA isolated from various grapevines, which were used in the experiments of transmission of 'Shiraz' disease (SD) from 'Cinsaut Blanc' clone P163/12 to SD-susceptible 'Merlot' and 'Shiraz' using mealybug Planococcus ficus and grafting were investigated. The amplicons were generated in RT-PCR based on virus-specific or random hexamers oligonucleotide primers. Standard molecular techniques and high-throughput sequencing (HTS), respectively, were applied. The results supported the hypothesis that GVA M5v variant present in 'Cinsaut Blanc' P163/12, which is a member of group II of GVA variants associated with SD, is crucial for developing this disease. HTS data did not reveal any other grapevine viruses besides GLRaV-3 and GVA in SD-affected grapevines, except for GVE which, however, was not present in all diseased plants.

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Published

2020-07-30

Issue

Vol. 59 No. 3 (2020): Vitis

Section

Article

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