Bacillus megaterium BMJBN02 induces the resistance of grapevine against downy mildew

Authors

  • Xueying Xie Shandong Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
  • Xiaoyang Han State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, China
  • Guangzhi Zhang Shandong Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
  • Susu Fan Shandong Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
  • Hongzi Zhou Shandong Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China
  • Xinjian Zhang Shandong Key Laboratory of Applied Microbiology, Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, China

DOI:

https://doi.org/10.5073/vitis.2022.61.101-109

Keywords:

grape downy mildew, Plasmopara viticola, Bacillus megaterium, induced systemic resistance, salicylic acid, pathogenesis-related genes

Abstract

Grape downy mildew caused by Plasmopara viticola is one of the most destructive diseases of grapes. All grape cultivars are susceptible to P. viticola. However, the resistance of grape plants could be induced in plant defense with some help of microbes. In this study, Bacillus megaterium BMJBN02 obtained from farmland soil was shown to regulate the resistance of grapevine against downy mildew. The salicylic acid (SA) content and the expression of pathogenesis-related (PR) genes of grapes under different treatments were examined using high-performance liquid chromatography-mass spectrometry (HPLC-MS) and reverse transcription- quantitative polymerase chain reaction (RT-qPCR), and it was found that SA content and the expression of PR genes could play a role in regulating the resistance of grapevine against downy mildew. The five-year plot experiment showed that the resistance effectiveness of isolate BMJBN02 was approximately equal to that of 0.1 % nicotinyl morpholine (commercial fungicide). Therefore, this study provides a valuable candidate method that uses B. megaterium BMJBN02 by regulating the resistance of grape against downy mildew for quality and yield of grape in commercial productivity.

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Published

2022-12-01

Issue

Vol. 61 No. 3 (2022): Vitis

Section

Article

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