Exogenous application of double-stranded RNA to reduce grapevine Pinot gris virus titre in in vitro grown Vitis vinifera

Authors

  • Kamalpreet Kaur School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, Australia.; Agriculture Victoria Research, Department of Energy, Environment and Climate Action, Melbourne, Victoria, Australia.
  • Amy Rinaldo The Australian Wine Research Institute, Adelaide, South Australia, Australia.
  • Brendan Rodoni School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, Australia.; Agriculture Victoria Research, Department of Energy, Environment and Climate Action, Melbourne, Victoria, Australia.
  • Fiona Constable School of Applied Systems Biology, La Trobe University, Bundoora, Victoria, Australia.; Agriculture Victoria Research, Department of Energy, Environment and Climate Action, Melbourne, Victoria, Australia.

DOI:

https://doi.org/10.5073/vitis.2024.63.06

Keywords:

RNAi, dsRNA, tissue culture, motifs, GPGV

Abstract

A method of delivering short synthetic double-stranded RNA (dsRNA) to stimulate RNA interference (RNAi)-mediated control for grapevine Pinot gris virus (GPGV) in grapevines was developed and evaluated in this study. The dsRNA molecule targeting the RNA-dependent RNA polymerase (RdRp) gene of the GPGV genome was designed and produced by a twostep polymerase chain reaction (PCR) approach followed by in vitro transcription of the amplicon. A significant decrease in virus titre was observed seven days after dipping shoot tips of GPGV-infected tissue culture (TC) plantlets into a solution of GPGV-RdRp-dsRNA followed by re-introduction to TC. The effect was more pronounced in shoot tips dipped in the GPGV-RdRp-dsRNA solution for 24 hours than in tips dipped for two hours. This study represents the first successful demonstration of dsRNA-mediated control in TC plantlets for GPGV and offers a promising avenue to provide virus-free material to nurseries, contributing to the overall health and sustainability of the viticulture industry.

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Published

2024-07-12

Issue

Vol. 63 (2024)

Section

Article

License

Copyright (c) 2024 The Author(s)

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