Characterization, functional validation and gene expression patterns of two 14-3-3 isoforms from Vitis vinifera

Authors

  • G. Farace Institut des Sciences de la Vigne et du Vin (ISVV), UMR EGFV, 210 Chemin de Leysotte, CS 50008, 33882 Villenave d’Ornon Cedex, France
  • S. Visconti Department of Biology, University of Rome “Tor Vergata” Rome, Italy
  • C. Marchive INRA-AgroParisTech, UMR1318, Versailles, France
  • P. Aducci Department of Biology, University of Rome “Tor Vergata” Rome, Italy
  • M. Marra Department of Biology, University of Rome “Tor Vergata” Rome, Italy
  • C. Trossat-Magnin Institut des Sciences de la Vigne et du Vin (ISVV), UMR EGFV, Villenave d’Ornon, France

DOI:

https://doi.org/10.5073/vitis.2015.54.33-40

Keywords:

14-3-3 proteins, Vitis vinifera

Abstract

In eukaryotic cells, 14-3-3 proteins constitute a family of conserved regulatory proteins which bind to a large number of phosphorylated partners. Although a great body of evidences accumulated in herbaceous species indicates that 14-3-3s play key roles in regulation of plant growth, information about function in tree plants is still very scarce. In this paper, we report the characterization of two novel 14-3-3s from grapevine (Vitis vinifera 'Cabernet Sauvignon') named VV1_CS and VV2_CS. The VV1_CS and VV2_CS cDNA were expressed in Escherichia coli and the recombinant proteins were shown to be functional proteins using plasma membrane H+-ATPase as target. Transcript analysis during grape development or under elicitor-induced stress revealed differential expression of the two 14-3-3 isoforms.



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Published

2015-03-01

Issue

Vol. 54 No. 1 (2015): Vitis

Section

Article

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