Characterization of polyphenolic metabolites in grape hybrids


  • Zhengchang Liang
  • Yingzhen Yang
  • Lailiang Cheng
  • Gan-Yuan Zhong



grapes, Vitis, , hybrids, polyphenols, secondary metabolites


Cultivated and experimental grape hybrids represent an important part of the Vitis germplasm for grape improvement. In this study, we characterized the composition and content of polyphenolic compounds in the berries of 48 hybrid grapes for two consecutive years. A total of 48 polyphenolic compounds, including 28 anthocyanins, 6 flavanols, 6 flavonols, 2 hydroxybenzoic acids and 6 hydroxycinnamic derivatives, were identified via HPLC-MS and quantified by HPLC-DAD. The content of total polyphenols as well as individual polyphenolic compounds varied significantly among grape hybrids. A number of grape hybrids with high content of total polyphenols and various individual groups of polyphenolic compounds were identified. Principal component analyses identified several polyphenolic compounds, significantly influencing the content variation of total polyphenols and individual groups of polyphenols. Plot analyses on the basis of PC1 and PC2 values provided some interesting insights into the genetic relationships among these grape hybrids. This work is an important addition to our ongoing effort in developing a comprehensive database of nutrition- and health-related secondary metabolites in the Vitis germplasm for future grape improvement.