Combining molecular and metabolomic analysis to evaluate transgenic <i>Vitis vinifera</i> plants expressing the <i>Vitreoscilla</i> haemoglobin (VHb)
DOI:
https://doi.org/10.5073/vitis.2013.52.77-84Keywords:
gene transfer, metabolomics, UPLC/MS, vhb gene, , Vitis vinifera, Vitreoscilla stercorariaAbstract
Transgenic V. vinifera 'Brachetto' plants expressing Vitreoscilla stercoraria haemoglobin gene (vhb) were obtained by transferring the pBI-VHb construct harbouring the vhb and the nptII genes, via A. tumefaciens EAH 105. From 18 distinct transgenic plantlets, 2 lines were randomly chosen and 11 and 14 plants respectively obtained by micropropagation were acclimated for 100 days in the greenhouse, together with 10 micropropagated plants of a wild-type control line. A data-driven assessment, aimed at evaluating all measurable lowmolecular weight organic compounds in these transgenic plants was performed using UPLC-Q-TOF with an ESI interface. Comparisons between controls and transgenic lines and between the two transgenic lines detected significant differences in some metabolic patterns. For each line, a list of identified compounds was extracted from the 100 most significant biomarkers.
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