Proteomic analysis of sex conversion induced by CPPU in male grapevine of <i>Vitis amurensis</i>
DOI:
https://doi.org/10.5073/vitis.2013.52.177-184Keywords:
Vitis amurensis Rupr, male grapevine, sex conversion, CPPU, proteomeAbstract
If N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) could induce sex conversion in male plants of Vitis amurensis Rupr., this would reduce blindness of selection for male parents according to the fruit characters in cold-tolerant and disease-tolerant grape crossbreeding. Flower bud samples of male plants were treated with 100 mg∙L-1 CPPU at 15 days before anthesis. Two-dimensional gel electrophoresis (2-DE) was used to analyze the proteins related to sex conversion at different development time points. More than 600 protein spots were detected. Among them, 31 differentially expressed proteins were identified by MALDI-TOF/TOF, and 24 protein spots could be assigned to a probable function. Seventeen proteins participated in the sex conversion and with complex interaction. Sex conversion might receive the ROS signal in the beginning, and then pollen tube proteins were proposed to down-regulate to repress the stamen development, while the up-regulated cell elongation protein might promote the development of pistil. Adenine phosphoribosyl transferase 3 was proposed as the key protein in the sex organ conversion that was up-regulated by CPPU in the male V. amurensis achieving the ability to fruit in the end.
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