Selection strategies for marker-assisted background selection with chromosome-wise SSR multiplexes in pseudo-backcross programs for grapevine breeding
DOI:
https://doi.org/10.5073/vitis.2013.52.193-196Keywords:
gene introgression, simulation study, multiplex PCR, microsatellite, marker-assisted selectionAbstract
Organizing SSR markers located on one chromosome into PCR multiplexes has the potential to reduce the costs of marker analysis. The optimal selection strategies for such chromosome-wise multiplexes have not yet been investigated. We investigated with computer simulations three different selection strategies for gene introgression with a pseudo-backcross scheme and a marker density of one marker every 10 cM. Selecting individuals with the highest number of chromosomes carrying V. vinifera alleles at all background marker loci reduced the number of required multiplexes by 7.24-7.87 % in generations pBC4-pBC6 for population sizes nt = 150-300 individuals per pseudo-backcross generation.
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