A simple, inexpensive and environmentally friendly method for high throughput DNA extraction from grapevine (<i>Vitis</i> spp.)

Authors

  • L. Lemke
  • M. Rex
  • E. Zyprian
  • R. Töpfer

DOI:

https://doi.org/10.5073/vitis.2011.50.7-10

Keywords:

DNA extraction, leaf, grapevine

Abstract

The extraction of DNA is a basic tool for molecular analyses, which is difficult for grapevine due to its content of secondary metabolites. In terms of purity and yield, DNA extraction kits are superior; however, they are also expensive. Here we describe an efficient protocol to re-use the DNeasy 96 Plant Kit from Qiagen GmbH (Hilden, Germany) by preparing homemade buffers and regenerating the commercial 96 column plates. Leaf tissue extractions of different grapevine samples resulted in genomic DNA with a high molecular weight and sufficient purity suitable for molecular studies like restriction assays or various amplification analyses. This high throughput method is especially economical and does not require the use of laboratory robotics for pipetting steps.

 

Downloads

Published

2015-04-01

Issue

Vol. 50 No. 1 (2011): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.