Activation of gene expression during hypersensitive response (HR) induced by auxin in the grapevine rootstock cultivar 'Börner'
Keywords:Vitis, phylloxera, hypersensitive response (HR), suppression subtractive hybridization (SSH), DNA-microarray
The cultivar ‘Börner’ is one of the very few grape phylloxera (Daktulosphaira vitifoliae Fitch) resistant rootstocks commercially available. In contrast to tolerant or sensitive rootstocks, ‘Börner’ roots react to grape phylloxera attack with a hypersensitive response leading to necroses around the puncture sites. In this study, we identified genes differentially up-regulated during the HR. HR was chemically induced in root cells by the application of indol-acetic-acid (IAA). After a cDNA subtraction of induced and non-induced material, the subtracted cDNA-samples were also hybridized to Arabidopsis microarray chips to identify differentially expressed candidate genes. The microarray data were analyzed and differentially expressed genes were grouped into different functional categories, e.g. signal transduction pathways, transcription factors, defence associated genes. Primers were designed to target genes of interest putatively involved in the HR. So far, 38 ESTs induced in ‘Börner’ roots undergoing a HR have been sequenced and annotated.
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