Cryopreservation of embryogenic cell suspensions of the Spanish grapevine cultivars ‘Albariño’ and ‘Tempranillo’

Authors

  • M. E. González-Benito
  • C. Martín
  • J. R. Vidal

DOI:

https://doi.org/10.5073/vitis.2009.48.131-136

Keywords:

cell cultures, desiccation, somatic embryogenesis, encapsulation, liquid nitrogen, Vitis vinifera

Abstract

Embryogenic cell suspensions of two elite Spanish grapevine cultivars (‘Albariño’ and ‘Tempranillo’) were successfully cryopreserved by encapsulation-dehydration. The method implied the encapsulation of cells in alginate beads, subsequent culture in liquid medium with 1 M sucrose for 4 days, and desiccation for 2-4 h in the flow of a laminar-flow bench, before immersing in liquid nitrogen. With this simple method, up to 50 % of cell viability in the cryopreserved beads was measured (using the triphenyl tetrazolium method), which corresponded to vigorous growth of 100 % of beads after culture on semi-solid medium. The cryopreserved encapsulated cells were successfully used for initiation of new cell suspensions and their embryogenic capacity was studied. This cryopreservation method is an advance to store ready-to-use competent embryogenic tissue for grapevine genetic transformation projects.

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Published

2015-04-08

Issue

Vol. 48 No. 3 (2009): Vitis

Section

Article

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