Evaluation of aluminium tolerance in grapevine rootstocks

Abstract

Aluminum (Al) toxicity is a major worldwide agricultural problem. At low pH, Al speciates into the soluble and phyto-toxic form Al³⁺, inhibiting the root growth and affecting plant development. In Brazil, agriculture in acidic soils with elevated concentration of Al has significantly increased in the last decades. Therefore, in order to achieve efficient agriculture practices, the selection of plant cultivars with improved Al resistance has become crucial in this type of soils. In this work we have evaluated the Al resistance of six genotypes of grapevine rootstocks. The grapevine hardwood cuttings were grown in nutrient solution in the absence and presence of 250 and 500 μM Al at pH 4.2. The phenotypic indexes of relative root growth, fresh and dry root weight, root area, hematoxylin staining profile, and Al content were evaluated for all six genotypes. These phenotypic indexes allowed us to identify the 'Kober 5BB', 'Gravesac', 'Paulsen 1103', and 'IAC 766' grapevine rootstocks genotypes as the ones with the highest resistance to Al. Likewise, 'IAC 572' and 'R110 genotypes were the most Al-sensitive cultivars. We evaluated the root organic acid exudation profile in the most Al-resistant ('Kober 5BB') and most Al-sensitive ('R110') in plantlets cultivated in vitro in the absence and presence of 100, 200, and 400 μM of Al. Among several compounds detected, citrate was the only organic acid related to the Al resistance phenotype observed in the 'Kober 5BB' genotype. The high constitutive citrate exudation observed in 'Kober 5BB' strongly suggests that exudation of this particular organic acid may impart Al-resistance/amelioration in grapevine.