Determination of gluconic acid in wine using high pressure liquid chromatography with pulsed amperometric detection
DOI:
https://doi.org/10.5073/vitis.2009.48.201-204Keywords:
wine, gluconic acid, HPLC-PADAbstract
The paper establishes a new HPLC method for measurement of the total content of gluconic acid (GA) in wine using a pulsed amperometric detector equipped with a gold electrode cell and an anion exchange column. A NaOH solution was used at a flow rate of 1.5 ml·min-1 as a mobile phase under isocratic conditions. Analysis took 30 min. The linearity of the method was shown up to 500 mg·l-1, with a LOQ of 2.0 mg·l-1 (10σ). Precision (as RSD%) was under 2.5 % in the usual range of GA concentration in natural wines. Accuracy was checked in comparison with the usual enzymatic method.
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