Inhibitors of neomycin phosphotransferase II enzyme-linked immunosorbent assay in grapevine (<i>Vitis vinifera</i> L.) leaves

Authors

  • S. H. Eom
  • B. I. Reisch

DOI:

https://doi.org/10.5073/vitis.2008.47.7-9

Keywords:

ELISA, ellagic acid, NPTII detection, polyphenols, tartaric acid, transgenic grapevine, Vitis vinifera

Abstract

Grapevine tissue extracts are rich in compounds that may inhibit detection and/or extraction of protein, DNA, and RNA. One such example can be found in the use of enzyme-linked immunosorbent assays (ELISA) to detect neomycin phosphotransferase II (NPTII) in leaf tissue. The objective of this study was to identify grape leaf components that interfere with protein detection via ELISA. A series of compounds were identified, and tartaric and ellagic acids were most inhibitory to NPTII detection. Polyphenolics as well as the low pH of grape leaf extracts also reduced the effectiveness of ELISA detection.


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Published

2015-04-09

Issue

Vol. 47 No. 1 (2008): Vitis

Section

Article

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