Comparison of different methods for SNP detection in grapevine

Authors

  • M. Troggio
  • G. Malacarne
  • S. Vezzulli
  • G. Faes
  • M. Salmaso
  • R. Velasco

DOI:

https://doi.org/10.5073/vitis.2008.47.21-30

Keywords:

grapevine, SNPs, SSCP, capillary electrophoresis, minisequencing, multiplex PCR

Abstract

Single nucleotide polymorphisms (SNPs) are the most abundant of all markers, both in animal and plant genomes. In crops and tree species considerable investment has been recently made on this genomic technology. While large-scale characterisation of SNPs by high-throughput techniques is possible, such highthroughput platforms are not available to all plant breeding laboratories. This report compares alternative multi-purpose and affordable methods for SNP assay in grapevine (Vitis spp.). In particular, the efficiency, sensitivity and reliability of single-strand conformation polymorphism (SSCP) on both non-denaturant gels and fluorescence-based capillary electrophoresis are compared with minisequencing (single nucleotide extension reaction). The results indicate that when multiplexing in combination with minisequencing is a mid-throughput, reliable and flexible technique for the detection of SNPs and can therefore be used effectively to improve marker assisted breeding in grapevine.

 

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Published

2015-04-09

Issue

Vol. 47 No. 1 (2008): Vitis

Section

Article

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