The use of phosphinothricin resistance as selectable marker for genetic transformation of grapevine

Authors

  • R. Jardak-Jamoussi
  • B. Bouamama
  • A. Mliki
  • A. Ghorbel
  • G. M. Reustle

DOI:

https://doi.org/10.5073/vitis.2008.47.35-37

Keywords:

Vitis vinifera, genetic transformation, Agrobacterium tumefaciens, bar, gus, , PPT

Abstract

A transformation procedure with the bar gene as a selectable marker was established via Agrobacterium-mediated transformation using strain LBA4404 harbouring the vector pPZP200-bar-gus-intron. Recreation of embryogenic cells from transformation stress in PPT free medium for four weeks improved viability and number of GUS expressing cells. Concentration of 2.5 mg·l-1 PPT yielded highest selection efficiency. Transgenicity of the regenerated grapevine plants was confirmed by histochemical GUS assay and bar specific PCR and RT/PCR. With the described procedure, 20 % of regenerated embryos could be converted into transgenic grapevines.

 

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Published

2015-04-09

Issue

Vol. 47 No. 1 (2008): Vitis

Section

Article

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