Isolation and characterization of cDNA encoding stilbene synthases from Chinese wild <i>Vitis pseudoreticulata</i>

Authors

  • Xiping Wang
  • Yuejin Wang
  • Chaohong Zhang
  • Junke Zhang

DOI:

https://doi.org/10.5073/vitis.2007.46.104-109

Keywords:

Vitis pseudoreticulata, stilbene synthase, mRNA differential display, RACE

Abstract

mRNA differential display was employed to study powdery mildew disease resistance gene expression in Chinese wild Vitis pseudoreticulata 'Baihe-35-1' inoculated with Erysiphe necator (syn. Uncinula necator) under natural field conditions. A cDNA fragment T11AC/B0320-723 showing homology to stilbene synthase (STS) gene expressed more strongly at 1, 3, 5, 7 and 9 days after inoculation of leaves than in controls was found. The full cDNA length was cloned by rapid amplification of cDNA ends (RACE). Sequencing of the full length cDNA revealed cDNA sequences, sized 1288, 1411, 1468, 1492, 1506 and 1556 base pairs encoding 6 homologous polypeptides with 392 amino acid residues each, that were designated as VpSTS1, VpSTS2, VpSTS3, VpSTS4, VpSTS5 and VpSTS6 respectively. The deduced amino acid sequences shared identity of 65 %, 77 % and more than 94 % with Pinus strobus STS, Vitis vinifera chalcone synthase (CHS), and Vitis riparia, Vitis labrusca, Parthenocissus henryana, Cissus rhombifolia, Parthenocissus quinquefolia and Vitis vinifera STS, respectively.

 

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Published

2015-04-14

Issue

Vol. 46 No. 3 (2007): Vitis

Section

Article

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