Different DNA extraction methods can cause different AFLP profiles in grapevine (<i>Vitis vinifera</i> L.)

Authors

  • A. Benjak
  • J. Konradi
  • R. Blaich
  • A. Forneck

DOI:

https://doi.org/10.5073/vitis.2006.45.15-21

Keywords:

DNA extraction, Vitis, AFLP-PCR

Abstract

Amplified fragment length polymorphism (AFLP) is widely used for DNA fingerprinting and it has been broadly applied in population genetics. Since it is based on restriction digestion and PCR-based amplification it can be influenced by different chemical compounds commonly found in the isolated DNA. DNA extraction procedures may alter the AFLP banding profiles through DNA quality. Hence the DNA extraction method is crucial to produce reproducible AFLP-banding profiles.
In this work two sets of AFLP analyses were performed on 62 Pinot noir, 6 Pinot blanc and 4 Pinot gris (Vitis vinifera L.) clones, and profiles obtained after three different DNA extraction methods were compared. AFLP profiles were different for the same genotypes due to the DNA extraction method used.

 

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Published

2015-04-14

Issue

Vol. 45 No. 1 (2006): Vitis

Section

Article

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