Grapevine virus C and grapevine leaf roll associated virus 2 are serologically related and appear to be the same virus

Authors

  • S. Masri
  • H. Rast
  • R. Johnson
  • P. Monette

DOI:

https://doi.org/10.5073/vitis.2006.45.93-96

Keywords:

GVC, GLaV2, grapevine viruses

Abstract

Protein extracted from grapevines infected with GLRaV-2 virus was subjected to electrophoresis, followed by Western blots. A protein band of about 23 kDa was detected in all infected plants. When GVC antibodies were used on blots obtained from the same infected plants, a similar protein band was detected in all infected plants. To address the possibility of the presence of another virus with the same molecular weight, the gene coding for the coat protein of GLRaV-2 was cloned and expressed in E. coli. The expressed protein reacted positively to both GLRaV- 2 and GVC antibodies. Using Immunosorbent Electron Microscopy (ISEM), polyclonal antibodies prepared against either GVC or GLRaV-2 trapped and decorated GLRaV-2 particles. The cDNA from GVC-infected grapevines and Nicotiana benthamiana were cloned and sequenced. All of the clones that were sequenced had the same sequence as GLRaV-2. Based on the data obtained, we concluded that GVC is the same virus as GLRaV-2.

Keywords:

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Published

2015-04-14

Issue

Vol. 45 No. 2 (2006): Vitis

Section

Article

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