Isolation and characterization of <i>Agrobacterium</i> strains from grapevines in Bulgarian vineyards and wild grapes, <i>V. vinifera</i> ssp. <i>silvestris</i>

Authors

  • I. Genov
  • I. Atanassov
  • I. Tsvetkov
  • A. Atanassov

DOI:

https://doi.org/10.5073/vitis.2006.45.97-101

Keywords:

Agrobacterium, grapevine, wild grapes, V. vinifera ssp. silvestris, pathogen strain, polymerase chain reaction

Abstract

Twenty strains of Agrobacterium spp. were isolated from grapevines derived from 9 different grape-growing regions in Bulgaria and from symptomless wild grapes, Vitis vinifera ssp. silvestris, naturally growing in forests of South-East Bulgaria. The isolated strains were examined by biochemical and pathogenicity tests and PCR analysis of chromosomal and plasmid-located genes. Two of the studied strains resemble non-pathogenic A. tumefaciens, but the others were determined as tumorigenic A. vitis. Only one out of 18 A. vitis strains, isolated from grapevines, was found to harbor vitopine Ti plasmid type. Surprisingly, all 4 A. vitis strains isolated from symptomless V. vinifera ssp. silvestris plants, proved to be tumorigenic. Thus, for the first time we report the isolation of pathogenic A. vitis strains from wild grapes. The strict correlation between the results from biochemical and virulence tests and PCR analysis suggests that the chosen PCR procedures can be used efficiently for direct PCR testing for the presence and tumorigenicity of A. vitis in grapevines.

 

Downloads

Published

2015-04-14

Issue

Vol. 45 No. 2 (2006): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.