Encapsulated somatic embryos of grape (<i>Vitis vinifera</i> L.): An efficient way for storage and propagation of pathogen-free plant material

Authors

  • D. K. Das
  • N. K. Nirala
  • M. K. Redoy
  • S. K. Sopory
  • K. C. Upadhyaya

DOI:

https://doi.org/10.5073/vitis.2006.45.179-184

Keywords:

encapsulation, germination, plantlet regeneration, somatic embryos

Abstract

Cotyledonary-stage somatic embryos (5-7 mm in length) originating from leaf explants of grape (Vitis vinifera L.) cv. Pusa seedless were encapsulated individually in 2 % alginate gel. The encapsulated somatic embryos (ESEs) germinated successfully on 0.7 % agar medium containing B5 macrosalts (half strength), MURASHIGE and SKOOG microsalts (full strength), 3 % sucrose and 2.9 μM gibberellic acid. The percentage of germination of ESEs was higher than that of nonencapsulated somatic embryos (NSEs) of the same size on the same medium. The percent germination of ESEs increased (69.2 ±2.8) on medium supplemented with quarter strength B5 macrosalts. Of the germinating ESEs, 36 % developed into plantlets. Abscisic acid at 0.004 and 0.02 μM had no significant influence on the frequency of germination and plantlet development, however resulted in a 4-week delay in germination. Transferring the embryos onto the full-strength B5 medium containing sucrose and ABA (0.04 μM) for 4-6 weeks prior to encapsulation resulted in extended storage of up to 90 d without loss of the germination potential and the capacity to regenerate into plantlets. Normally developed plantlets regenerated from ESEs were successfully adapted to soil.

 

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Published

2015-04-14

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