Somatic embryogenesis from stem nodal sections of grapevine

Authors

  • P. Maillot
  • F. Kieffer
  • B. Walter

DOI:

https://doi.org/10.5073/vitis.2006.45.185-189

Keywords:

Vitis vinifera, grapevine, axillary bud, nodal explant, somatic embryogenesis

Abstract

Indirect somatic embryogenesis was obtained for 11 clones of 6 Vitis vinifera cultivars: Cabernet-Sauvignon, Chardonnay, Gewürztraminer, Grenache, Merlot and Sauvignon, and for the rootstock Fercal [(Vitis berlandieri x Vitis colombard) x (Vitis vinifera x Vitis berlandieri)], starting from vegetative explants of in vitro plantlets. Embryogenic callus was recovered from nodal explants of every tested clone, while leaf explants led to embryogenesis only for the rootstock Fercal. We thus showed that axillary bud microcuttings are valuable explants for inducing somatic embryogenesis in V. vinifera and Fercal. Embryogenic cell lines have been maintained through secondary embryogenesis, and some embryos were converted into whole plantlets. A complete protocol for somatic embryogenesis and plant regeneration was therefore designed, using this very simple method.

 

Downloads

Published

2015-04-14

Issue

Vol. 45 No. 4 (2006): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.