Characterization of Agrobacterium tumefaciens strains isolated from grapevine

Authors

  • E. Szegedi
  • S. Bottka
  • J. Mikulás
  • L. Otten
  • S. Sule

DOI:

https://doi.org/10.5073/vitis.2005.44.49-54

Keywords:

Agrobacterium vitis, crown gall, opine, tartrate-utilization, Ti plasmids, Vitis vinifera

Abstract

Grapevine crown gall is predominantly induced by Agrobacterium vitis, but rarely, Agrobacterium tumefaciens may also cause this disease. We have collected 18 grapevine isolates of A. tumefaciens from distinct geographical regions and various grapevine varieties. Characterization of these strains showed that 7 of the 18 strains carried A. vitis-type octopine/cucumopine Ti plasmids, utilized tartrate, were avirulent on pea and did not respond to the virC-specific PCR primers like A. vitis. Eleven of the 18 strains carried A. tumefaciens-type Ti plasmids, did not grow on tartrate, were virulent on pea and reacted positively to virC-specific PCR primers. Two of these strains induced octopine/agropine-, and two strains induced agropine producing tumors on grapevine and/or Kalanchoe tubiflora plants. The remaining 7 strains showed the characteristic properties of A. tumefaciens nopaline strains and were sensitive to agrocin 84.


Downloads

Published

2015-04-15

Issue

Vol. 44 No. 1 (2005): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.