Genetic diversity among twelve grape cultivars indigenous to the Carpathian Basin revealed by RAPD markers
DOI:
https://doi.org/10.5073/vitis.2005.44.87-91Keywords:
RAPD, Vitis vinifera L., Carpathian-Basin, genetic diversity, cultivar identificationAbstract
Twelve cultivars (Vitis vinifera L.) were subjected to RAPD analysis in order to estimate the genetic diversity among these genotypes and to analyse their genetic relationships. The study was performed using 28 primers that generated 120 polymorphic fragments. There was genetic variation among the cultivars with values of genetic diversity ranging from 0.419 to 0.642 using the Jaccard coefficient. UPGMA analysis of distance matrix resulted in a dendrogram with three clusters. The dendrogram shows that the cultivars of our study can be distinguished to a relatively high degree. Results were compared with the taxonomic classification and with the synonyms of the cultivars. The RAPD technique was useful for identification and discrimination of these grape cultivars.
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