ISSR-PCR for assessment of genetic relationships among grape varieties cultivated in India

Authors

  • V. M. Dhanorkar
  • S. A. Tamhankar
  • S. G. Patil
  • V. S. Rao

DOI:

https://doi.org/10.5073/vitis.2005.44.127-131

Keywords:

genetic relationships, grapes, ISSR, Vitis

Abstract

Genetic relationships among 43 varieties cultivated in India were characterized using ISSR PCR. Out of total 139 reproducible fragments generated by 13 informative primers, 96 were polymorphic. The similarity coefficient ranged from 0.65 to 0.96. Cluster analysis resulted in the formation of two main clusters, consisting mainly of Vitis labrusca and V. vinifera types. One single variety belonging to V. rotundifolia James grouped with V. labrusca types but separated initially from them. Two varieties, Lake Emerald and Muscat, were completely outgrouped. Varieties belonging to V. vinifera appeared to be more diverse and separated further into many subgroups. In contrast, V. labrusca types were homogeneous. The results showed that ISSR is an efficient and reliable marker system for genetic analysis of grape cultivars.


Downloads

Published

2015-04-16

Issue

Vol. 44 No. 3 (2005): Vitis

Section

Article

License

The content of VITIS is published under a Creative Commons Attribution 4.0 license. Any user is free to share and adapt (remix, transform, build upon) the content as long as the original publication is attributed (authors, title, year, journal, issue, pages) and any changes to the original are clearly labeled. We do not prohibit or charge a fee for reuse of published content. The use of general descriptive names, trade names, trademarks, and so forth in any publication herein, even if not specifically indicated, does not imply that these names are not protected by the relevant laws and regulations. The submitting author agrees to these terms on behalf of all co-authors when submitting a manuscript. Please be aware that this license cannot be revoked. All authors retain the copyright on their work and are able to enter into separate, additional contractual arrangements.