Molecular characterization of officially registered Sangiovese clones and of other Sangiovese-like biotypes in Tuscany, Corsica and Emilia-Romagna

Authors

  • I. Filippetti
  • C. Intrieri
  • M. Centinari
  • B. Bucchetti
  • C. Pastore

DOI:

https://doi.org/10.5073/vitis.2005.44.167-172

Keywords:

Vitis vinifera, Sangiovese, microsatellite (SSR), amplified fragment length polymorphism (AFLP), cultivar, clone

Abstract

The present study was designed (1) to identify and determine the origin of the genetic variability via SSR and AFLP within a group of 39 Sangiovese clones officially listed in the National Grapevine Registry, (2) to pinpoint varietal differences and potential family relations among 34 Sangiovese-like biotypes, via the SSR markers. Most biotypes are regarded as Sangiovese but sometimes are known under different names. In both studies the reference standard was the registered Sangiovese clone SG 12T.
No polymorphism was found among the officially listed 39 clones analysed at 6 microsatellite loci. This enabled us to confirm their origin from a single mother plant, thereby supporting the view that any morphological or qualitative differences may be the result of propagation-related mutagenic events. A subsequent AFLP analysis of 26 of the 39 clones showed polymorphic bands in three of them (two identical) that may correspond to a mutagenic event.
Assays with SSR markers on 34 Sangiovese-like biotypes collected in Tuscany, Corsica and Emilia-Romagna showed that 28 are identical with the reference Sangiovese clone SG 12T, while the remaining 6 (Sangiovese 1, Sangiovese 6, Morellino, Poverina, Sangiovese forte and Brunellone) are genetically different from SG 12T and among one another so that no direct family relations could be established.


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Published

2015-04-16

Issue

Vol. 44 No. 4 (2005): Vitis

Section

Article

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